谁能帮我翻译成英文啊，急急急

谁能帮我中文翻译成英文(合同)~

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Chinese food, long history. In etiquette, pay attention to Food is the country where eating etiquette naturally become an important part of food culture. With the constant exchange of Western food culture, more and more foreigners are also popular. Chinese food banquet ritual known as Duke began thousands of years of evolution, of course, there will be no "Meng Guang Liang Hong received the case " that day, but it was finally formed a set of widely accepted today, eating dining etiquette, is Eating etiquette in ancient inheritance and development. Etiquette for eating Chinese food feast of the nature, purpose and different; different regions, but also different. Chinese food dishes are cups, plates, bowls, plates, chopsticks, spoons and six. In the formal dinner, glass dish on the top left, the glass on the upper right. Chopsticks and spoon can be placed on specific transposon, or on the paper sleeve. Communal chopsticks and spoons on the best on the specific transposon. Chinese food is generally the order of the meal: the first on the cold, after the hot dishes, sweets and fruit on the last. Before dinner, the waiter sent to each of the first with a wet towel is a towel, it is best not to use it to wipe her face. In the shrimp, crab, chicken and other dishes before, the waiter will give her a little spittoon, in which floating lemon slice twist or rose petals, it is not drink, but hand-washing purposes. Wash your hands, you can turn his hands dampened fingers, gently rinsed, and then with a small towel.

Pick to: purpose: to construct CEA single antibody-garlic acid enzyme gene expression vectors and eukaryotic fusion in pichia pastorios in expression. Method: according to the garlic acid enzyme gene sequence design upstream and downstream primers, introduced respectively EcoRI and XbaI extinction enzymes site. To preserve the pGEMP-T-Alliinasee plasmid for template for PCR amplification. Extracted from e. coli pPIcz α C plasmid, then, PCR products and pPIcz α C plasmid respectively with EcoRI and XbaI cut the enzyme, the construction of the pPIcz α C-Alliinase restructuring plasmid. To preserve pGEMP-T-CEA as templates to design primer, introduced respectively XhoI and EcoRI extinction enzymes site, PCR product, will PCR products and pPIcz α C-Alliinase respectively with EcoRI and XhoI cut the enzyme, the construction of the pPIcz α C-Alliinase-CEA restructuring plasmid. The enzyme cut, PCR identification and sequencing analysis. Will the right pPIcz identification α C-Alliinasee-CEA plasmid electricity transformation to pichia pastorios to express, sds-page electrophoresis and Western Blotting testing purpose the protein expression. Results: 1. The successful construction CEA single antibody-garlic acid enzyme gene fusion eukaryotic expression plasmid, about the size of for 5600 bp. 2. In pichia pastorios fusion protein get preliminary expression, molecular weight is about 78 KD. Conclusion: CEA single antibody-garlic acid enzyme gene fusion can get in pichia pastorios preliminary expression.

Key word: carcinoma antigen embryo; Garlic acid enzyme; Single antibody; Gene expression
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Abstract: Objective: to construct CEA scFv antibody-conjugated alliinase fusion gene eukaryotic expression vector and expression in Pichia pastoris expression. Methods: according to the design of alliinase gene sequences upstream and downstream primer, respectively, the introduction of EcoRI and XbaI restriction endonuclease sites. For the preservation of the pGEMP-T-Alliinasee plasmid as template for the PCR amplification. From Escherichia coli extracts pPIcz alpha C plasmid, then, PCR product and pPIcz alpha C plasmid respectively by EcoRI and XbaI double enzyme, pPIcz alpha C-Alliinase recombinant plasmid construction. In order to save pGEMP-T-CEA as template, redesigning primer, respectively, introduce XhoI and EcoRI restriction endonuclease sites, PCR products, the PCR product and pPIcz alpha C-Alliinase respectively by EcoRI and XhoI double digestion, constructing pPIcz alpha C-Alliinase CEA recombinant plasmid. Double enzyme, PCR identification and sequencing analysis. The identification of the correct pPIcz alpha C-Alliinasee-CEA plasmid transformation to the Pichia pastoris expression, SDS-PAGE electrophoresis and Western Blotting detection target protein expression. Results: the successful construction of single chain antibody1CEA alliinase fusion gene eukaryotic expression plasmid, size of about5600bp. The 2fusion protein in Pichia pastoris gets preliminary expression, molecular weight of about78KD. Conclusion: CEA single chain antibody of alliinase gene fusion can be obtained in Pichia pastoris expression.

Keywords: carcinoembryonic antigen; garlic; antibody; gene expression

Objective: to construct CEA single antibody-garlic acid enzyme gene expression vectors and eukaryotic fusion in pichia pastorios in expression. Method: according to the garlic acid enzyme gene sequence design upstream and downstream primers, introduced respectively EcoRI and XbaI extinction enzymes site. To preserve the pGEMP-T-Alliinasee plasmid for template for PCR amplification. Extracted from e. coli pPIcz α C plasmid, then, PCR products and pPIcz α C plasmid respectively with EcoRI and XbaI cut the enzyme, the construction of the pPIcz α C-Alliinase restructuring plasmid. To preserve pGEMP-T-CEA as templates to design primer, introduced respectively XhoI and EcoRI extinction enzymes site, PCR product, will PCR products and pPIcz α C-Alliinase respectively with EcoRI and XhoI cut the enzyme, the construction of the pPIcz α C-Alliinase-CEA restructuring plasmid. The enzyme cut, PCR identification and sequencing analysis. Will the right pPIcz identification α C-Alliinasee-CEA plasmid electricity transformation to pichia pastorios to express, sds-page electrophoresis and Western Blotting testing purpose the protein expression. Results: 1. The successful construction CEA single antibody-garlic acid enzyme gene fusion eukaryotic expression plasmid, about the size of for 5600 bp. 2. In pichia pastorios fusion protein get preliminary expression, molecular weight is about 78 KD. Conclusion: CEA single antibody-garlic acid enzyme gene fusion can get in pichia pastorios preliminary expression.

Key word: carcinoma antigen embryo; Garlic acid enzyme; Single antibody; Gene expression

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