那位好心人帮我翻译中文摘要 非常急谢谢!!!!
Solves the thyroxine acceptor β△ gene in big mouse different organization distributed characteristic, the understanding real-time fluorescence quota PCR principle and the application method. Uses real-time fluorescence quota PCR familiar the method analysis result. Method: TRβ△ the gene has the expression in many kinds of organizations, the liver, the brain, the skeleton muscle, the heart, the kidney expression are most. Withdraws the big mouse liver, the brain, the skeleton muscle, the heart, the kidney and so on many place organization's mRNA, withdraws mRNA is the template counter duplication synthesizes the cDNA first chain. And increases double strand cDNA take it as template PCR. According to the goal gene which copies designs two to direct the thing. Uses real-time fluorescence quota PCR the method to carry on the quantitative analysis. Finally: 1. the extraction organizes total RNA, OD260/OD280 to be situated between 1.7-2.0, OD260/230 is situated between 1.5-1.9. After formaldehyde denatured agarose gel electrophoresis, 28S and 18S density ratio approximately 2. 2. the agarose gel electrophoresis appraises PCR to increase the result: 1) GAPDH the PCR product's agarose gel electrophoresis appraisal result showed: Has the goal about anticipated 160bp approximately to increase the product belt. 2) TRβΔ the PCR product's agarose gel electrophoresis appraisal result showed: The DNA molecular weight size which increases after PCR is the 176bp goal banding. 3) reorganizes the clone material particle pGEM- gene the appraisal result: By EcoRⅠCarries on the enzyme to the reorganization material particle to cut, 2% agarose gel electrophoresis examination enzyme cuts the result, may see about the 3000bp material particle fragment, as well as with insertion sequence size match case another DNA banding. The goal gene and the housekeeping gene quantitative determination, it increases the curve and the curve of solubility has not seen the mixed peak, simultaneously melts the tune prominent peak correspondence Tm value with to increase the product theory Tm to be close. Conclusion: The findings showed that TRβ△ the gene has certain spatial distribution characteristic in the mRNA level's expression: The liver, the brain, the spleen, in the kidney organization has this gene expression, and in spleen's expression quantity is higher than other organizations obviously.
1st, 凯里 city apple mountain park construction detailed plan instruction booklet abstract: The apple mountain park is 凯里 in a city city green system important constituent. This project is for the purpose of through carries on the overall plan coordination to in the park each kind of present situation natural resource and the artificial environment, to the park in the road traffic system, the public service facilities, the botanical garden green and the municipal administration infrastructure carries on the synthesis layout, achieved the plan designs and develops the management control the desired effect, guarantees the park development construction the economic society rationality and the feasibility. Through the plan and the plan implementation, completes take the natural ecology and the humanities landscape perfect union, the collection tour, the ecology, the leisure, the popular science as a body city comprehensive park, through protects aspect the and so on city landscape, ecology, leisure, traveling coordinated development, satisfies the people leisure entertainment, watches the amusement and rest, the healthy body exercise, the popular science study demand. Key word: Comprehensive park; Construction detailed plan; Ecology; National culture; The amusement and rest environment 2, the politics passes the newly developed area construction detailed plan abstract: The politics passes the newly developed area construction detailed plan is a proper attention to both traveling landscape and the housing property development comprehensive project, divides into two issues to carry on the construction, an issue of plan already completed, this plan for two issue of constructions detailed plan. The project travels the small town including the national character and style, the synthesis traveling necessary service area, as well as sports leisure subject park newly developed area three subjects contents. This plan objective for causes the politics to pass the newly developed area to become with the movement base match subject newly developed area; Fully creates "运动型", "the scientific health" using the sports leisure movement culture characteristic and "art" the newly developed area; Cultivates the positive good neighborhood relations, has "the social life" richly the newly developed area. The ultimate objective is causes the plan politics to pass the newly developed area to become spiritual the homeland, the mind native land, the green world, the fresh source occupies, the city breath, has the new times to plan the community. Key word: The politics passes the newly developed area; Construction detailed plan; Community; Subject park; Traveling landscape 3, Guizhou Province 绥阳 county Zheng Changzhen plot construction detailed plan abstract: In line with the social efficiency, the economic efficiency, the environment benefit organic unification principle, will argue vigorously this community plan construction for 绥阳 the county future the modernized life community, will be full of the energy the person will occupy the environment model district. This plan the plot construction uses the scale, the function localization to Zheng Changzhen, to use to assign, the plan structure carries on the detailed strict proof, and comprehensively considers this plot and the urban environment fusion as well as the plot own overall image and 可识别性, proper attention to both general character and individuality. At the same time, this plan to the plot construction landscape style, the square construction, the road system, for the drainage system, the electric power telecommunication project system and so on the necessary facility system construction carries on the careful complete plan, strives for this plot construction is a collection education, the culture, the entertainment, the trade is a body comprehensive demonstrative housing plot.
In-depth development of a market economy, on the one hand for the survival and development of enterprises to provide more and broader space, on the other hand so that competition among enterprises become more intense. Some enterprises have suffered because of mismanagement or external factors lead to poor cash flow, profitability declined, insolvent, a debt trap, leading to debt restructuring activities continue to occur. Debt restructuring in resolving the debt risks, adjust the structure of property rights, and optimize the capital structure, re-integration of economic resources, promote enterprises in the legal system on the track of sustainable development, playing an important role.Economic development of the direct impact of accounting, primarily reflected in the formulation of accounting rules. 2006 amendments to the debt restructuring of the accounting standards in terms of the 01 criteria have many reasonable, in line with the objective reality, more scientific, it's perfect, of China's socialist market economic development, mature play an important role. But at the same time, corporate accounting standards due to the limited development, determine its formulation and implementation is a gradual process, can not be accomplished overnight, to be gradually improving. Therefore, the corporate debt restructuring through the theoretical study, not only for debt restructuring to provide reference for the development, accelerate the improvement of accounting standards and development, but also will promote the accounting and capital markets, and other areas of research. Therefore, the research paper on China's accounting standards building work is of important significance, but also for the securities market regulators also have important reference value.
As China's accounting standards have been given the function of regulating corporate behavior, the current accounting standards building relatively speaking, still lags behind the development of capital markets. How reasonable and feasible to use 06 criteria for debt restructuring, debt restructuring how to regulate accounting and information disclosure, as 06 after the promulgation of guidelines for the priority issues. In addition, the study of a specific accounting standards contribute to the amendment of the current situation of China's capital market to provide more ice on standardized ideas. Based on this starting point of this paper that the 06 criteria in the practical application of certain problems still exist, the need to continue to study and perfect. Therefore, on practical issues of corporate debt restructuring and 06 accounting standards effective operation is the purpose of this paper.
This paper studies the main objectives are: First, on debt restructuring of financial theories, models and evaluation of the reorganization, debt restructuring state-owned enterprises in the corresponding problem of the 06 debt restructuring Accounting Standards Amendment analysis and evaluation; three testing debt restructuring Practical application of accounting standards; four in the previous study on the basis of corresponding measures.
In the introduction to some of the major topics of this paper described the background of domestic and foreign debt restructuring theoretical research the status quo, the purpose and significance, the main content and methods. In addition to Introduction, this paper is divided into three chapters elaborate, the main contents of the chapters are as follows:
Chapter 1 is the full text of some of the basic theory, described in the basic theory of corporate debt restructuring on the basis of the current corporate debt restructuring of China's basic mode of the corresponding analysis and evaluation, China's state-owned enterprises and restructuring the debt status of an objective analysis, Provides a study of China's debt restructuring financial and accounting issues ideas and methods
The second major chapter on debt restructuring to improve accounting standards and improve the conduct a detailed analysis, beginning with the revised guidelines launched on background, on this basis, 06 and 01 criteria for debt restructuring guidelines, analysis, evaluation, on the basis of New guidelines in the process of implementation of the problem was analyzed.
Chapter III of the study at this stage of China's enterprises favorable conditions for debt restructuring and debt restructuring for state-owned enterprises and 06 guidelines for the implementation of the existing problems, given the corresponding recommendations. Debt restructuring that is reasonably determine the scope and object; improve corporate governance. Optimization of internal accounting environment to build an active market for the widespread use of fair value and create the conditions for the establishment of appropriate intermediaries, to strengthen the law enforcement agencies to assess just, fair, a recognized credit rating system so that the fair value of assets and access to debt assessment Solve problems; clear debt restructuring of the regulatory bodies and the terms of reference; improve the overall quality of accounting staff, as soon as possible to 06 criteria.
累死我了/呵呵
★人生在勤,不索何获--张衡
★业精于勤而荒于嬉,行成于思而毁于随 --韩愈
★天才就是无止境刻苦勤奋的能力 --卡莱尔
★聪明出于勤奋,天才在于积累 --华罗庚
★好学而不勤问非真好学者。
★书山有路勤为径,学海无涯苦作舟。
★勤劳一日,可得一夜安眠;勤劳一生,可得幸福长
眠 --达·芬奇(意大利)
★形成天才的决定因素应该是勤奋 -郭沫若
★人的大脑和肢体一样,多用则灵,不用则废 -茅以升
★你想成为幸福的人吗?但愿你首先学会吃得起苦 --屠格涅夫
★灵感不过是“顽强的劳动而获得的奖赏” --列宾
★贵有恒何必三更眠五更起,最无益只怕一日曝十日寒。
★成功=艰苦劳动+正确方法+少说空话 --爱因斯坦
★不经历风雨,怎能见彩虹 -《真心英雄》
★宝剑锋从磨砺出,梅花香自苦寒来。
★自古以来学有建树的人,都离不开一个“苦”字。
★天才就是百分之九十九的汗水加百分之一的灵感 --爱迪生
★艺术的大道上荆棘丛生,这也是好事,常人望而却步,只有意志坚强的人例外 --雨果
--------------------------------------------------
(转贴)
天才不是别的,而是辛劳和勤奋 —— 比丰
谨慎的勤奋带来好运 —— 英国
科学是为了那些勤奋好学的人,诗歌是为了那些知识渊博的人 —— 约瑟夫·鲁
没有人会因学问而成为智者。学问或许能由勤奋得来,而机智与智慧却有懒于天赋 —— 约翰·塞尔登
没有任何动物比蚂蚁更勤奋,然而它却最沉默寡言 —— 富兰克林
勤奋的人是时间的主人,懒惰的人是时间的奴隶 —— 朝鲜
勤奋和智慧是双胞胎,懒惰和愚蠢是亲兄弟 —— 民谚
天才绝不应鄙视勤奋 —— 小普林尼
勤奋者废寝忘食,懒惰人总没有时间 —— 日本
智慧源于勤奋,伟大出自平凡 —— 民谚
我在科学方面所作出的任何成绩,都只是由于长期思索、忍耐和勤奋而获得的 —— 达尔文
合抱之木,生于毫末;九层之台,起于垒土;千里之行,始于足下。
--------------老聃 引自《老子·道德经》
骐骥一跃,不能十步;驽马十驾,功在不舍;锲而舍之,朽木不折;锲而不舍,金石可镂。
--------------荀况 引自《荀子,劝学》
天行健,君子以自强不息。
--------------《周易·乾》(秦汉前儒家经典)
流水不腐,户枢不蠹,民生在勤。
--------------张少成
勤能补拙是良训,一分辛劳一分才。
--------------华罗庚
在艺术上我决不是一个天才。为了探求精深的艺术技巧,我曾在苦海中沉浮,渐渐从混沌中看到光明。苍天没有给我什么独得之厚,我的每一步前进,都付出了通宵达旦的艰苦劳动和霜晨雨夜的冥思苦想。
--------------范曾(当代书画家)
聪明在于勤奋,天才在于积累。
--------------华罗庚
古往今来,凡成就事业,对人类有所作为的,无不是脚踏实地,艰苦登攀的结果。
--------------钱三强(当代物理学家)
我们每个人手里都有一把自学成才的钥匙,这就是:理想、勤奋、毅力、虚心和科学方法。
--------------华罗庚
攀登科学高峰,就像登山运动员攀登珠穆朗玛峰一样,要克服无数艰难险阻,懦夫和懒汉是不可能享受到胜利的喜悦和幸福的。
--------------陈景润(当代数学家)
对搞科学的人来说,勤奋就是成功之母!
--------------茅以升(现代桥梁工程专家)
科学的灵感,决不是坐等可以等来的。如果说,科学上的发现有什么偶然的机遇的话,那么这种“偶然的机遇”只能给那些有素养的人,给那些善于独立思考的人,给那些具有锲而不舍的精神的人,而不会给懒汉。
--------------华罗庚转引自1980年5月20日《文汇报》
即使天才,在生下来的时候第一声啼哭,也和平常的儿童一样,决不会就是一首好诗。
--------------鲁迅引自《未有天才之前》
才华是刀刃,辛苦是磨刀石,很锋利的刀刃,若日久不用磨,也会生锈,成为废物。
--------------老舍(现代作家、艺术家)
回答者:幸福飞过海 - 经理 五级 10-17 22:26
业精于勤荒于嬉,行成于思毁于随(韩愈)
“天才就是这样,终身劳动,便成天才。”(门捷列夫)
“天才是百分之一的灵感,百分之九十九的血汗”(爱迪生)
“科学的灵感,决不是坐等可以等来的。如果说,科学上的发现有什么偶然的机遇的话,那么这种‘偶然的机遇’只能给那些学有素养的人,给那些善于独立思考的人,给那些具有锲而不舍的精神的人,而不会给懒汉。”(华罗庚)
“古今中外,凡成就事业,对人类有作为的无一不是脚踏实地、艰苦攀登的结果。”(钱三强)
“攀登科学高峰,就象登山运动员攀登珠穆朗玛峰一样,要克服无数艰难险阻,懦夫和懒汉是不可能享受到胜利的喜悦和幸福的。”(陈景润)
回答者:李泽尘 - 初入江湖 三级 11-20 00:41
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我总结的一句话: 分数是聪明和高智商的象征,这虽不是真理,但却是现实。
回答者:匿名 11-20 20:32 对最佳答案的评论:
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